Sialic acid aldolase (NPL)
CAt#
CBGEN00008
Abbr
NPL, Recombinant (K-12 MG1655)
Source
E. coli
Species
K-12 MG1655
Enzyme Commission Number
4.1.3.3
Molecular Weight
33 KDa
Purity
min 95% by SDS-PAGE
Physical Form
Supplied as a lyophilized powder
Package
10U,50U,250U
Description
Catalyzes the pyruvate and N-acetylmannosamine to form N-acetylneuraminic acid (sialic acid).
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the formation of1 μmol Sia from ManNAc and pyruvate per minute at 37 °C.
References
Chemoenzymatic synthesis of CMP-sialic acid derivatives by a one-pot two-enzyme system: comparison of substrate flexibility of three microbial CMP-sialic acid synthetases. Yu Hai et. al. Bioorganic & Med. Chem. 12 (24), 6427-6435, 2004