Endo-β-N-acetylglucosaminidase A (Endo-A)
CBGEN00015
Abbr
Endo-A, Recombinant (Arthrobacter protophormia)
Source
E. coli
Species
Arthrobacter protophormia
Enzyme Commission Number
3.2.1.96
Molecular Weight
69 KDa
Purity
min 95% by SDS-PAGE
Physical Form
Supplied as a lyophilized powder
Package
200U,500U,1000U
Description
An Endoglycosidase is an enzyme that releases oligosaccharides from glycoproteins or glycolipids. It may also cleave polysaccharide chains between residues that are not the terminal residue, although releasing oligosaccharides from conjugated protein and lipid molecules is more common.
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the release of 1 nmol N-glycan from RNaseB per minute at 37 °C.
References
1. Endo-β-N-acetylglucosaminidase, an enzyme involved in processing of free oligosaccharides in the cytosol. Tadashi Suzuki et. al. PNAS, 99(15), 9691-9696, 2002; 2. Induction and Purification of Endo-β-N-Acetylglucosaminidase from Arthrobacter protophormiae Grown in Ovalbumin. Kaoru Takegawa et.al. Applied and Environmental Microbiology. 3107-3112, 1989