1,3-HexNAc transferase (LgtA)

CBGEN00024

Abbr

LgtA, Recombinant (Neisseria meningitides)

Source

E. coli

Species

Neisseria meningitides

Enzyme Commission Number

2.4.1.150

Molecular Weight

--

Purity

min 95% by SDS-PAGE

Physical Form

Supplied as a lyophilized powder

Package

1u,5u,10u

Description

A β-1,3-N-Acetyl-Hexosaminyl-transferase is an enzyme that catalyzes the chemical reaction: UDP-N-acetyl-D-glucosamine + β-D-galactosyl-(1→4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-β-D-glucosaminyl-(1→3)-β-D-galactosyl-(1→4)-N-acetyl-D-glucosaminyl-R.

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of Galβ1,3Lac-NAc from UDP-GlcNAc and LacNAc per min at 37°C.

References

Synthesis of unnatural sugar nucleotides and their evaluation as donor substrates in glycosyltransferase-catalyzed reactions. Khaled A. et. al. Carbohydr Res 339 (16), 2641-2649, 2004