1,3-HexNAc transferase (LgtA)
CBGEN00024
Abbr
LgtA, Recombinant (Neisseria meningitides)
Source
E. coli
Species
Neisseria meningitides
Enzyme Commission Number
2.4.1.150
Molecular Weight
--
Purity
min 95% by SDS-PAGE
Physical Form
Supplied as a lyophilized powder
Package
1u,5u,10u
Description
A β-1,3-N-Acetyl-Hexosaminyl-transferase is an enzyme that catalyzes the chemical reaction: UDP-N-acetyl-D-glucosamine + β-D-galactosyl-(1→4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-β-D-glucosaminyl-(1→3)-β-D-galactosyl-(1→4)-N-acetyl-D-glucosaminyl-R.
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of Galβ1,3Lac-NAc from UDP-GlcNAc and LacNAc per min at 37°C.
References
Synthesis of unnatural sugar nucleotides and their evaluation as donor substrates in glycosyltransferase-catalyzed reactions. Khaled A. et. al. Carbohydr Res 339 (16), 2641-2649, 2004