beta1,3-GalNAc transferase (LgtD)
CBGEN00025
Abbr
LgtD, Recombinant (Neisseria meningitides)
Source
E. coli
Species
Neisseria meningitides
Enzyme Commission Number
2.4.1.-
Molecular Weight
--
Purity
min 95% by SDS-PAGE
Physical Form
Supplied as a lyophilized powder
Description
A β-1,3-GalNAc transferase is an enzyme that catalyzes the chemical reaction: UDP-N-acetyl-D-galactose + D-galactose = UDP + N-acetyl-β-D-galacosaminyl-(1→3)-β-D-galactose.
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of GalNAcβ1,3-Gal from UDP-GalNAc and Gal per min at 37°C.
References
1. Large scale in vivo synthesis of globotriose and globotetraose by high cell density culture of metabolically engineered Escherichia coli. Antoine T. et. al. Biochimie. 87(2), 197-203, 2005; 2. Donor Substrate Regeneration for Efficient Synthesis of Globotetraose and Isoglobotetraose. Jun Shao. et. al. Appl Environ Microbiol 68(11), 5634-5640, 2002.
Package
1u,5u,10u