α2,3-sialyltransferase (PmST1)
CBGEN00002
Abbr
PmST1, recombinant Pasteurella multocida
Source
E. coli
Species
Pasteurella multocida
Enzyme Commission Number
2.4.99.4
Molecular Weight
46 KDa
Purity
min 95% by SDS-PAGE
Physical Form
Supplied as a lyophilized powder
Description
Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides.
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol Siaα2,3Lac from CMP-Sia and Lactose per minute at 37 °C.
References
1. Cyclooxygenase-2 enzyme induces the expression of the α-2,3-sialyltransferase-3 (ST3Gal-I) in breast cancer. Daisy Sproviero et.al The Journal of biological chemistry, 287(53), (2013); 2. Chemoenzymatic synthesis of mono- and di-fluorinated Thomsen-Friedenreich (T) antigens and their sialylated derivatives. Jun Yan et. al Organic & biomolecular chemistry, 11(5), (2012); 3. Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism. Di Mo et. al Molecular biology of the cell, 23(18), (2012)