β1,4-galactosyltransferase (LgtB)
CBGEN00005
Abbr
LgtB, Recombinant (Neisseria meningitides)
Source
E. coli
Species
Neisseria meningitides
Enzyme Commission Number
2.4.1.90
Molecular Weight
34 KDa
Purity
min 95% by SDS-PAGE
Physical Form
Supplied as a lyophilized powder
Package
1U,5U,25U
Description
A alpha-N-acetylgalactosaminyltransferase is an enzyme that catalyzes the chemical reaction: UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose → UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose.
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the formation of1 μmol Gal1,4GlcNAc from UDP-Gal and GlcNAc per minute at 37 °C.
References
Efficient Preparation of Natural and Synthetic Galactosides with a Recombinant β-1,4-Galactosyltransferase-/UDP-4‘-Gal Epimerase Fusion Protein. Ola Blixt et. al. J. Org. Chem. 66 (7), 2442-2448, 2001