β1,4-galactosyltransferase (LgtB)

CBGEN00005

Abbr

LgtB, Recombinant (Neisseria meningitides)

Source

E. coli

Species

Neisseria meningitides

Enzyme Commission Number

2.4.1.90

Molecular Weight

34 KDa

Purity

min 95% by SDS-PAGE

Physical Form

Supplied as a lyophilized powder

Package

1U,5U,25U

Description

A alpha-N-acetylgalactosaminyltransferase is an enzyme that catalyzes the chemical reaction: UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose → UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose.

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the formation of1 μmol Gal1,4GlcNAc from UDP-Gal and GlcNAc per minute at 37 °C.

References

Efficient Preparation of Natural and Synthetic Galactosides with a Recombinant β-1,4-Galactosyltransferase-/UDP-4‘-Gal Epimerase Fusion Protein. Ola Blixt et. al. J. Org. Chem. 66 (7), 2442-2448, 2001