CMP-sialic acid synthetase (NmCSS)
Cat#
CBGEN00009
Abbr
NmCSS, Recombinant (Neisseria meningitides)
Source
E. coli
Species
Neisseria meningitides
Enzyme Commission Number
2.7.7.43
Molecular Weight
26 KDa
Purity
min 95% by SDS-PAGE
Physical Form
Supplied as a lyophilized powder
Package
10U,50U,100U
Description
Catalyzes the pyruvate and N-acetylmannosamine to form N-acetylneuraminic acid (sialic acid).
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol CMP-Sia from Sialic acid and CTP per minute at 37 °C.
References
Chemoenzymatic synthesis of CMP-sialic acid derivatives by a one-pot two-enzyme system: comparison of substrate flexibility of three microbial CMP-sialic acid synthetases. Hai Yu et. al. Bioorganic & Med. Chem. 12 (24), 6427-6435, 2004