N-acetylhexosamine 1-kinase (NahK)
CBGEN00011
Abbr
NahK, Recombinant (Bifidobacterium longum)
Source
E. coli
Species
Bifidobacterium longum
Enzyme Commission Number
2.7.1.162
Molecular Weight
40 KDa
Purity
min 95% by SDS-PAGE
Physical Form
Supplied as a lyophilized powder
Package
5U,20U,100U
Description
This enzyme catalyses the following chemical reaction: ATP + N-acetyl-D-hexosamine ADP + N-acetyl-alpha-D-hexosamine 1-phosphate. This enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway in the probiotic bacterium Bifidobacterium longum.
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the formation of1 μmol of GlcNAc-1-P from GlcNAc and ATP per minute at 37 °C.
References
1. Substrate promiscuity of N-acetylhexosamine 1-kinases. Li Y. et. al. Molecules. 16 (8), 6396-6407, 2011; 2. Identification of N-Acetylhexosamine 1-Kinase in the Complete Lacto-N-Biose I/Galacto-N-Biose Metabolic Pathway in Bifidobacterium longum. Mamoru Nishimoto et. al. Appl Environ Microbiol 73 (20), 6444-6449, 2007.