GlcNAc-1-P uridyltransferase (GlmU)
CBGEN00012
Abbr
GlmU, Recombinant (E. coli K-12)
Source
E. coli
Species
E. coli K-12
Enzyme Commission Number
2.3.1.157
Molecular Weight
25 KDa
Purity
min 95% by SDS-PAGE
Physical Form
Supplied as a lyophilized powder
Package
5U,20U,100U
Description
N-Acetylglucosamine-1-phosphate uridyltransferase (GlmU), a bifunctional enzyme exclusive to prokaryotes, belongs to the family of sugar nucleotidyltransferases (SNTs). The enzyme binds GlcNAc-1-P and UTP, and catalyzes a uridyltransfer reaction to synthesize UDP-GlcNAc, an important precursor for cell-wall biosynthesis.
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol UDP-GlcNAc from GlcNAc-1-P and UDP per minute at 37 °C.
References
1. GlmU (N-acetylglucosamine-1-phosphate uridyltransferase) bound to three magnesium ions and ATP at the active site. Vithani N. et. al. Acta Crystallogr F Struct Biol Commun. 70 (Pt6) 703-708, 2014; 2. Dissection of the Bifunctional Escherichia coli N-Acetylglucosamine-1-phosphate Uridyltransferase Enzyme into Autonomously Functional Domains and Evidence That Trimerization Is Absolutely Required for Glucosamine-1-phosphate Acetyltransferase Activity and Cell Growth. Frederique Pompeo et. al. J. Bio. Chem. 276 (6), 3833-3839, 2001; 3. Enzymatic synthesis of UDP-GlcNAc/UDP-GalNAc analogs using N-acetylglucosamine 1-phosphate uridyltransferase (GlmU). Wanyi Guan et. al. Chem. Comm. 6976-6978, 2009