α-1,3/4-fucosyltransferase
CBGEN00021
Abbr
1,3/4FucT, Recombinant (Helicobacter mustelae)
Source
E. coli
Species
Helicobacter mustelae
Enzyme Commission Number
2.4.1.65
Molecular Weight
--
Purity
min 95% by SDS-PAGE
Physical Form
Supplied as a lyophilized powder
Package
10u,50u,100u
Description
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of Fucα1,3/4glycans from GDP-Fuc and receptor per minute at 37 °C.
References
1. α1,4-Fucosyltransferase Activity: A Significant Function in the Primate Lineage has Appeared Twice Independently. Fabrice Dupuy. et. al. Molecular Biology and Evolution, 19 (6), 2002 2. Human alpha 1,3/4 fucosyltransferases. Characterization of highly conserved cysteine residues and N-linked glycosylation sites. Holmes EH. et. al. J. Boil. Chem. 275(32), 24237-24245, 2000.