1,4-N-acetylgalactosaminyl transferase; CgtA

CBGEN00030

Abbr

CgtA

Source

E. coli

Species

Campylobacter jejuni

Enzyme Commission Number

2.4.1.92

Molecular Weight

--

Purity

min 95% by SDS-PAGE

Physical Form

Supplied as a lyophilized powder

Package

1U,5U,10U

Description

GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively.

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol GalNAc beta 1,4 glycans from UDP-GalNAc and receptors per minute at 37 °C.

References

Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a. Ola Blixt. et. al. Carbohydr. Res. 340(12), 1963-1972, 2005.